This course will provide a basic training in IHC theory and practise to those working within the NHS, higher education institutions or within industry. The course will teach you the knowledge and confidence to manipulate basic IHC techniques to fit applications within your workplace.
On completion of the course, students will understand:
The complex interactions of an antigen and antibody and how this impacts on immunohistochemistry reactions
Mathematics relating to molarity and ratios
On completion of the course, students will be able to:
Apply a sound theoretical approach to the application of immunohistochemistry within the work environment
Carry out immunohistochemistry protocols to demonstrate how proteins are distributed within a tissue section
Identify positive staining within a tissue section using a light microscope
Discuss the interaction of an antigen and antibody and how this impacts on the immunohistochemistry reactions
Explain why the use of control samples are important.
Prepare reagents and antibodies including calculation of molarity and dilutions
Use histology laboratory equipment safely
Complete a risk assessment for a piece of equipment and input a chemical onto a COSHH form